Kód: 06822152
GFP is widely used as a §biological marker because of its superb fluorescence §properties. We have synthesized GFP chromophore and §its derivatives, and their photochemistry §and photophysics were investigated using various §stead ... celý popis
Nákupem získáte 181 bodů
GFP is widely used as a §biological marker because of its superb fluorescence §properties. We have synthesized GFP chromophore and §its derivatives, and their photochemistry §and photophysics were investigated using various §steady-state and time-resolved techniques. §We first compared their optical properties between §solution and solid state. We found some O-alkyl §GFP chromophore derivatives exhibit large §fluorescent enhancement in the solid state.§Cis/trans isomerization is thought to be responsible §for blinking behavior in fluorescent protein; §however, the mechanism of the thermal reverse §isomerization is more problematic. Using §Hammett plot studies, we conceived, §for the first time, a novel nucleophilic §addition/elimination mechanism. §Finally, a hydroxyl substituent at the meta position §shows enhanced charge transfer and greater acidity §in the excited state. As a result, we have §demonstrated that the fast quenching of the excited §state by internal conversion to the ground state is §much slower in meta- than in para-HOBDI derivatives. §This allows studies of this ultrafast intermolecular §ESPT that competes with isomerization.
Zařazení knihy Knihy v angličtině Mathematics & science Chemistry Organic chemistry
1806 Kč
Osobní odběr Praha, Brno a 12903 dalších
Copyright ©2008-24 nejlevnejsi-knihy.cz Všechna práva vyhrazenaSoukromíCookies
Nákupní košík ( prázdný )