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Více informací o knize Signal Transduction - Single Cell Techniques

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Anotace knihy

A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.

Parametry knihy

Zařazení knihy Knihy v angličtině Mathematics & science Science: general issues Scientific equipment, experiments & techniques

• Plný název: Signal Transduction - Single Cell Techniques
• Autor: Bert, van Duijn, Anneke Wiltink
• Jazyk: Angličtina
• Vazba: Brožovaná
• Počet stran: 468
• EAN: 9783642489761
• ISBN: 3642489761
• ID: 02012187
• Nakladatelství: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
• Hmotnost: 969 g
• Rozměry: 246 × 189 × 27 mm
• Datum vydání: 10. July 2012

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