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Anotace knihy

An indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. The techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation.Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal its unique structural features and biochemical functions. In p53 Protocols, Sumitra and Swati Palit Deb have assembled an indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. Described by leading basic and clinical researchers who have successfully used the methods, the techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation. Special effort has been made to discuss problems and their solutions to help ease the learning curve when standardizing a new method.§Diverse and highly practical, p53 Protocols offers both beginning and experienced researchers in cancer biology a gold-standard compendium of readily reproducible techniques for studying p53 and related proteins.

Parametry knihy

Zařazení knihy Knihy v angličtině Mathematics & science Biology, life sciences Cellular biology (cytology)

• Plný název: p53 Protocols
• Autor: Sumitra Deb, Swati Palit Deb
• Jazyk: Angličtina
• Vazba: Brožovaná
• Počet stran: 280
• EAN: 9781617373619
• ISBN: 1617373613
• ID: 01432015
• Nakladatelství: Humana Press Inc.
• Hmotnost: 494 g
• Rozměry: 227 × 152 × 19 mm
• Datum vydání: 28. October 2010

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