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Více informací o knize Hydrocarbon and Lipid Microbiology Protocols

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Anotace knihy

This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented. Tens of thousands of hydrocarbons, their derivatives and lipids play vital roles in all organisms, from structural cellular components to signalling molecules. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. Springer Protocols provide readily reproducible methods essential to research in hydrocarbon and lipid microbiology. Each chapter explains the principles underlying the method, followed by step-by-step protocols. Helpful notes provide important tips that will help circumvent common problems, and thus increase experimental success. §

Parametry knihy

Zařazení knihy Knihy v angličtině Mathematics & science Biology, life sciences Microbiology (non-medical)

• Plný název: Hydrocarbon and Lipid Microbiology Protocols
• Podnázev: Biochemical Methods
• Autor: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales Fernández
• Jazyk: Angličtina
• Vazba: Pevná
• Počet stran: 188
• EAN: 9783662491355
• ISBN: 3662491354
• ID: 02862917
• Nakladatelství: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
• Hmotnost: 526 g
• Rozměry: 189 × 259 × 17 mm
• Datum vydání: 27. August 2016

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