Mohlo by se vám také líbit

Více informací o knize Cell-free expression systems for disulfide rich proteins

• Parametry knihy
• Anotace
• Oblíbené z jiného soudku

Nákupem získáte 243 bodů

Anotace knihy

Most extracellular proteins are stabilized by multiple disulfide bonds formed by the oxidation of pairs of cysteine residues. The most popular host for recombinant protein production is Escherichia coli, but disulfide rich proteins are here often misfolded, degraded, or found in inclusion bodies. An alternative expression system is the cell-free in vitro translation system based on wheat germ extract. It is an open system in which an optimized mix of thiol-disulfide oxidoreductases or chaperones can be added, so that disulfide rich proteins have no chance to aggregate the moment their polypeptide chain emerges from the ribosomes. We have challenged this in vitro translation system with three disulfide rich proteins: mouse Found in Inflammatory Zone (mFIZZ1), Androctonus australis hector (AahII) scorpion toxin, and mouse Vascular Endothelial Growth Factor (mVEGF164). By co-translation of the thiol/disulfide oxidoreductase hQSOX1b, soluble and biological active protein were obtained.

Parametry knihy

Zařazení knihy Knihy v angličtině Mathematics & science Biology, life sciences

• Plný název: Cell-free expression systems for disulfide rich proteins
• Autor: Gad Wael
• Jazyk: Angličtina
• Vazba: Brožovaná
• Počet stran: 200
• EAN: 9783639666847
• ISBN: 3639666844
• ID: 09091824
• Nakladatelství: Scholars' Press
• Hmotnost: 299 g
• Rozměry: 229 × 152 × 12 mm
• Datum vydání: 14. October 2014

Oblíbené z jiného soudku

---

---

---