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Anotace knihy

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

Parametry knihy

Zařazení knihy Knihy v angličtině Technology, engineering, agriculture Biochemical engineering Biotechnology

• Plný název: Directed Evolution
• Podnázev: Methods and Protocols
• Autor: Andrew Currin, Neil Swainston
• Jazyk: Angličtina
• Vazba: Brožovaná
• Počet stran: 278
• EAN: 9781071621547
• ID: 43475465
• Nakladatelství: Springer, Berlin
• Rozměry: 254 × 178 mm
• Datum vydání: 06. July 2023

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