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This volume explores recent progress in the microelectronics arena, theresultant miniaturization of component device features to nanometersize particles, and the ensuing growth in the development and use ofmicrochip-based techniques in leading laboratories around the world.While the area of microelectronics is faced with daunting challenges,it promises fascinating new advances that may translate into productssuch as sensors and devices that use nanometer-sized building blocksfor real-world applications. The contributors to this volume are partof growing interdisciplinary efforts among pioneers inmicroelectronics, nanoscience, and health that will continue to createexciting new microchip-based applications across a variety of sectors.In the 16 chapters of this volume, leading scientists presenttechnically precise microchip-based assays for a variety ofapplications.Highlights include: methods for microchip electrophoresis for DNAseparation; fabrication of porous polymer monoliths in microfluidicchips for selective nucleic acid concentration and purification; rapidelectrical lysis of bacterial cells in a microfluidic device; anon-chip bioassay that uses immobilized sensing bacteria in athree-dimensional microfluidic network; and impedimetric detection forDNA hybridization within microfluidic biochips. Molecular diagnosticand immunologic protocols include microchip-based enumeration of humanwhite blood cells; microchip diagnosis of cervical cancer; protocolsfor DNA microchips toward molecular signatures in cervical cancer; amicrochip based assay for Interleukin-6; and methods formicrochip-based electrochemical enzyme immunoassays. Several chaptersare devoted to topics in microarray technology, including: applicationsof functional protein microarrays; allergen microarrays for thediagnosis of specific IgE in a multiplex biochip-based immunoassay;protein-array based multiplexed cytokine assays; and lectin microarraysfor glycoprotein assays.This cutting-edge book explores recent progress in the microelectronics arena. It examines the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. In the sixteen chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications.Microchip-Based Assay Systems explores recent progress in the microelectronics arena, the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. While the area of microelectronics faces daunting challenges, the field also promises fascinating new advances that may translate into products such as sensors and devices that use nanometer-sized building blocks for real-world applications. The contributors to this volume are part of growing interdisciplinary efforts among pioneers in microelectronics, nanoscience, and health that will continue to create exciting new microchip-based applications across a variety of sectors. In the 16 chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications. The protocols detailed in this volume will help foster interest in microchip-based assays by providing readers with all the tools necessary to create their own microchip-based assays targeted to new applications. Certainly, Microchip-Based Assay Systems will help to expand the scope and accelerate the transition of microchip-based assays from academic and industrial research and development centers to real-world use.

Parametry knihy

Zařazení knihy Knihy v angličtině Medicine Pre-clinical medicine: basic sciences Medical genetics

• Plný název: Microchip-Based Assay Systems
• Podnázev: Methods and Applications
• Autor: Pierre N. Floriano
• Jazyk: Angličtina
• Vazba: Brožovaná
• Počet stran: 229
• EAN: 9781617376627
• ISBN: 1617376620
• ID: 01432248
• Nakladatelství: Humana Press Inc.
• Hmotnost: 421 g
• Rozměry: 229 × 152 × 13 mm
• Datum vydání: 28. October 2010

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